JSPK v0.92 by Jesper Søndergaard Pedersen

(Jesper’s SpektraMAX Plate Kinetics fitter)

 

JSPK is a program that allows fitting of kinetic data from SpectraMAX plate-readers to user-defined functions using the Levenberg-Marquardt least squares method. The main advantages of this program are:

• “Effortless” importing of data generated by SoftMAX.
• Rapid GUI for evaluation of wavelength combinations for noise reduction.
• Fitting to user-defined functions of up to 8 variables with graphical representation of starting parameters.
• Real-time residual plotting for fit evaluation.
• Seamless export of raw/reduced data and fitting results to spreadsheet programs.

 

Screenshot below shows the noise-reduced tryptophan fluorescence of four replicate wells during fibrillation of 0.14 g/l glucagon in 50mM glycine/NaOH pH 9.5.

The black line is a plot of the starting parameters seen on the top right panel.

 

This program relies heavily on the following delphi units:

• TPMATH (Delphi version DMATH) by Dr. Jean Debord.
• TPARSER by Renate Schaaf, Alin Flaider and Stefan Hoffmeister.
• ZOOMLER by Alexander Rublinetsky.

 

Download latest public version.

Read Instructions for use.

If you use this program then please cite the following article

Contact me: jsp@phage.dk

 

Legal mumbo-jumbo - Disclaimer

 

The software and accompanying instructions are provided "as is" without warranty of any kind. The authors do not warrant, guarantee, or make any representations regarding the use, or the results of the use of the software or accompanying instructions in terms of correctness, accuracy, reliabilty, currentness or otherwise. The entire risk as to the results and performance of the software is assumed by you. If the software or instructions are defective, you, and not the authors, assume the entire cost of all necessary servicing, repair or correction.

JSPK is copyrighted software. You may use and distribute it free of charge, but it must not be sold or offered as an inducement to buy other products.

 

Version history

 

Build 050221 - First public release

 

Build 050303 - Fixed program to be independent on the type of decimal separator used on a PC.

 

Build 050603 - Quick fix to allow importing wavescan as well as kinetic data.

 

Build 050701 - Fixed progress indicator window for importing SM data

 

Build 050706 - Fixed a number of graph scaling and fit boundary problems created in build 050602

 

Build 051214 - Implemented MaxDec:=1, to limit the maximum number of decimals on graph to one. Changed web-page reference to http://jspk.phage.dk in the About-box.

 

Build 060406 -  Fixed importing of '#Low' data - this is simply set to 1. Improved graph routine winplot used - removed maxdec:=1

 

Build 060418 -  Changed import TRichEdit to TMemo to prevent random import errors. Apparently text files are not loaded correct by TRichEdit even though PlainText:=True